INLIGHT® Kit

Glycans participate in a large number of cellular, molecular, and biological processes and are implicated in a number of diseases (e.g., Alzheimer’s, cancer). To aid the identification and relative quantification of glycans by LC-MS, Eurisotop offers the innovative INLIGHT^® (Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tag) glycan-tagging kit. This kit employs both natural (NAT) and stable isotope-labeled (SIL; ¹³C₆) phenyl 2-GPN reagents in the hydrazide derivatization of free glycans. While this tagging strategy was developed for N-glycans, it has also been adapted to O-glycan and heparin oligomer profiles. The INLIGHT kit contains five vials of NAT reagent and five vials of SIL reagent, which in total provides sufficient tagging for approximately 125 relative quantification experiments. The user manual provides step-by-step instructions for executing the INLIGHT strategy using maltoheptaose and fetuin A as examples. Data processing and analysis of derivatized glycans can be facilitated in GlycoHunter or Skyline.